Table of Contents
- 1 Does carbolfuchsin stain acid fast negative cells?
- 2 Why is carbol fuchsin used in acid-fast staining?
- 3 Are acid-fast negative cells stained by carbolfuchsin if so how can this be a differential stain quizlet?
- 4 What is acid fast staining technique?
- 5 What property of Nigrosin makes the negative stain work?
- 6 What type of staining is negative staining simple or differential?
- 7 How are acid fast negative cells stained by carbolfuchsin?
- 8 Is the acid fast stain the same as the Gram stain?
Does carbolfuchsin stain acid fast negative cells?
Are acid-fast negative cells stained by carbolfuchsin? Yes they are initially stained by carbolfuchsin but the decolorizing step, acid alcohol, removes stain from acid fast negative cells while acid fast positive cells retain the stain.
How is the acid fast stain a differential stain?
The acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium . The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms.
Why is carbol fuchsin used in acid-fast staining?
It is commonly used in the staining of mycobacteria as it has an affinity for the mycolic acids found in their cell membranes. Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells wall lipids than in the acid alcohol.
Is a negative stain a differential stain?
India ink is a negative stain that colors the background brown, leaving the cells bright and visible. A differential staining technique is a procedure that allows the observer to visually distinguish between different types of bacterial cells based on the idea that not all cell types stain equally.
Are acid-fast negative cells stained by carbolfuchsin if so how can this be a differential stain quizlet?
are acid fast negative cells stained by carbolfuchsin? if so can this be a differential stain? Yes it can be a differential stain because the carbolfuchsin is easily removed from the walls of the acid fast negative cells, but is locked into the mycolic acid fast positive cells.
What is the primary stain in the kinyoun acid-fast stain How does this differ from the primary stain in the Ziehl neelsen method?
The Kinyoun staining procedure is often referred to as cold carbolfuchsin because no heat is applied during the staining process unlike the Ziehl- Neelsen procedure. The primary stain for the Kinyoun procedure is the aniline dye, basic fuchsin, that stains all the cells present red.
What is acid fast staining technique?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
What color will an acid fast organism stain in the acid fast stain?
red
Acid fast stains are used to differentiate acid fast organisms such mycobacteria. Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the Kinyoun stain.
What property of Nigrosin makes the negative stain work?
Nigrosin is an acidic stain. This means that the stain readily gives up a hydrogen ion and becomes negatively charged. Since the surface of most bacterial cells is negatively charged, the cell surface repels the stain.
What is the primary stain in the kinyoun acid-fast stain?
The primary stain for the Kinyoun procedure is the aniline dye, basic fuchsin, that stains all the cells present red. The unique ability of mycobacteria to resist decolorization by acid-alcohol is why they are termed acid-fast, and will keep their red coloration throughout the staining process.
What type of staining is negative staining simple or differential?
Cards
| Term Which type of staining (negative, simple, differential) is better for looking at size? | Definition Negative staining |
|---|---|
| Term What are two examples of differential staining? | Definition gram stain and acid fast stain |
What type of staining is negative staining?
Negative staining requires an acidic dye such as India Ink or Nigrosin. India Ink or Nigrosin is an acidic stain. This means that the stain readily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged.
How are acid fast negative cells stained by carbolfuchsin?
Are acid-fast negative cells stained by carbolfuchsin? If so, how can this be a differential stain? Yes they are initially stained by carbolfuchsin but the decolorizing step, acid alcohol, removes stain from acid fast negative cells while acid fast positive cells retain the stain.
How can this be a differential stain?
If so, how can this be a differential stain? Yes they are initially stained by carbolfuchsin but the decolorizing step, acid alcohol, removes stain from acid fast negative cells while acid fast positive cells retain the stain. Why do you suppose the acid-fast stain is not as widely used as the Gram stain?
Is the acid fast stain the same as the Gram stain?
Yes they are initially stained by carbolfuchsin but the decolorizing step, acid alcohol, removes stain from acid fast negative cells while acid fast positive cells retain the stain. Why do you suppose the acid-fast stain is not as widely used as the Gram stain? When is it more useful than the Gram stain?
How are acid fast organisms resistant to disinfectants?
Acid-fast organisms are highly resistant to disinfectants and dry conditions. Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall.